![dmpc pro 1.03 dmpc pro 1.03](https://www.researchgate.net/profile/Maria-Hrmova/publication/298248338/figure/fig1/AS:339752051068934@1458014609839/Wheat-Germ-Cell-Free-Protein-Synthesis-of-Bot1-and-Expression-of-Bot1-in-P-pastoris-A_Q320.jpg)
Finally, we extend these studies to the plasma membranes of commonly used neuroblastoma, HeLa and fibroblast cells.
![dmpc pro 1.03 dmpc pro 1.03](https://i1.rgstatic.net/publication/236049009_Egg_Consumption_Modulates_HDL_Lipid_Composition_and_Increases_the_Cholesterol-Accepting_Capacity_of_Serum_in_Metabolic_Syndrome/links/00b4952240198a785a000000/largepreview.png)
Diffusion measurements and the subsequent FCS diffusion law analyses at different temperatures show that the modulation in membrane dynamics at high temperature (313 K) is a cumulative effect of domain melting and rigidity relaxation. The FCS diffusion law, a novel membrane heterogeneity ruler implemented in ITIR-FCS, is applied to show that the domains in the S o–L d phase are static and large while they are small and dynamic in the L o–L d phase. We show that the temperature dependence of membrane lateral diffusion, which is parameterized by the Arrhenius activation energy ( E Arr), can resolve the sub-resolution phase behavior of lipid mixtures. In this study, imaging total internal reflection fluorescence correlation spectroscopy (ITIR-FCS) is applied to monitor the diffusion within supported lipid bilayers (SLBs) as functions of temperature and composition. The characteristic lipid packing in these phases results in significant differences in their respective lateral dynamics. The NR pro les have been o set in the y-axis by an order of magnitude and SLD pro les o set in the y-axis by 5 10 6 A 2, for clarity. The abundance of the L o phase is assumed to be a consequence of the interaction between cholesterol and the other lipids, which are otherwise in either the L d or gel (S o) phase. The NR and SLD pro les at a surface pressure of (a) 25 mNm 1 for two contrasts of DMPC, and (b) 15 mNm 1 for two contrasts of DPPC. This is more obvious in the Arrhenius plot shown in Fig. The mobility jumped significantly once the T m is crossed.
![dmpc pro 1.03 dmpc pro 1.03](https://patentimages.storage.googleapis.com/0f/ab/c8/38528ad31d78ad/US20040029100A1-20040212-D00009.png)
The organization of the plasma membrane is regulated by the dynamic equilibrium between the liquid ordered (L o) and liquid disordered (L d) phases. RhoPE labeled DMPC bilayers (T m 296 K) shows two phases within the working temperature range (292313 K).The data in Table 1 corresponds to temperatures above T m.The phase transition can be clearly observed in Fig.